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Work experience

Researcher/ Ph.D. student

from 02.2014 to now (10 years 3 months)
Taras Shevchenko National University of Kyiv, Київ (Біотехнологія, медицина, фармакологія, клінічна діагностика)

-- Acquired proficiency in method of recombinant protein production Mammalian tyrosyl-tRNA synthetase using E.coli strain.
-- Researched conformation change of Mammalian tyrosyl-tRNA under different temperature conditions.
-- Acquired proficiency in modeling of rat esophagus alkaline burn injuries.
-- Researching of cytokine panel change, biochemical parameters and histology under chemical burn injuries of rats esophagus and their treatment.
-- Acquired proficiency in method of production of aqueous extract of Phaseolus vulgaris pods.

Education

Taras Shevchenko National University of Kyiv

RC "Institute of High Technology”, Department of Molecular biotechnology and bioinformatics, Київ
Higher, from 2014 to 2016 (1 year 11 months)

Coursework: Analysis of hydrophobic regions in eukaryotic Tyrosyl-tRNA synthetase using fluorescent probes 1,8-ANS and bis-ANS

Knowledge and skills

Language proficiencies

English — average

Additional information

PROFESSIONAL SKILLS:

Microbiological:
•Preparation of culture media
•Inoculation of bacterial culture
•Isolation of one single purified culture (Streaking)
•Transfection
•Specimen Preparation for Microscopy
•Bright-field, phase-contrast and electron microscopy

Immunological:
•ELISA

Molecular biology:
•Protein isolation and purification
•Gel filtration
•Membrane filtration
•Dialysis
•SDS-PAGE
•Western blot
•Bacterial protein expression
•Preparative column chromatography
•Affinity chromatography

Сhemical analysis:
•X-Ray Fluorescence Spectrometry
•Spectrophotometry
•Thin-layer chromatography (TLC)

Other:
GMP, GLP, GDP.

Membership of professional societies:
•Since February 2017 - Member of Ukrainian Biochemical Society (UBS), which is the member of the Federation of European Biochemical Societies (FEBS)

Organization skills:
-University сourse leader.
-Participated in annual international conference organization team (Shevchenko’s Spring).
-Conducted student training in biotechnology.
-Volunteered for school general education lections.

Driver license: B category (2012)

Hobbies: Object-oriented programming, cutting-edge technologies.